Studies on Oxidative Phosphorylation

Studies on the Mechanism of Oxidative Phosphorylation

Kinetic and nucleotide binding studies have shown that submitochondrial particles from bovine heart possess three exchangeable binding sites for ADP or GDP. In order of decreasing affinity at neutral pH, these sites will be referred to as sites I, II, and III, and their respective dissociation constants as KI, KIr, and KIII. In oxidative phosphorylation experiments in the presence of saturating...

Further studies on oxidative photosynthetic phosphorylation.

Studies on the kinetic mechanism of oxidative phosphorylation.

The kinetics of the synthesis of ATP from ADP and Pi by beef heart submitochondrial particles were examined. When Pi was the variable substrate positive cooperativity was observed, whereas if ADP was varied, linear double reciprocal plots were obtained. The analog of Pi, thiophosphate, was a noncompetitive inhibitor of ATP synthesis with respect to ADP, while the analog of ADP, AMP (CH2)P, was ...

Studies of factors involved in oxidative phosphorylation.

The oxidation of substrates of the citric acid cycle in mitochondria is coupled with the phosphorylation of ADP to ATP.I Considerable progress has been made in our understanding of the three electron transport reactions between DPNH and oxygen (DPNH -.cytochrome b -. cytochrome c -° 02), which are associated with the phosphorylation mechanism.2 In several laboratories3-7 actively phosphorylatin...

Effect of progesterone on oxidative phosphorylation.

A comparison of the effect of progesterone and DNP’ in accelerating ATPase activity in fresh rat liver mitochondria has indicated that the two may act, in a similar manner (1). Since the striking effect, of DNP on oxidative phosphorylation is probably related to its effect on mitochondrial ATPase (2, 3), an examination of the effect of progesterone on oxidative phosphorylation has been carried ...